Dissociated Tissue & Tumor Cells (DTCs)

Accelerate your precision medicine programs with our biomarker characterized dissociated tumor cells.

From the team who first validated cryopreserved tissue dissociations as a viable alternative to fresh cancer tissue samples for research applications.

Discovery’s expert cell services laboratory has evaluated DTCs for many applications through both internal R&D and client partnerships, cultivating unmatched expertise in applying cancer tissue dissociations to custom projects.

DTCs Product Details

  • Guaranteed Cell Counts - Our Dissociated Tumor Cells (DTCs) and other tissue dissociations are delivered as cryopreserved vials of single cell suspensions with guaranteed cell counts following our Thawing Viable Cell Products protocol.
  • Tumor, Immune & Stromal Cells – The final tissue dissociation vial will contain ALL of the various cell populations present in the tumor microenvironment.
  • Proprietary Custom Protocols – With proprietary custom protocols, our scientists have consistently minimized the effects of cryopreservation and demonstrated high percentages of viable cells both pre-freeze (81.3%) and post-thaw (69.7%).
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An ideal alternative to fresh tissues without the associated logistical challenges

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High cell viability pre-freeze and post-thaw

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Mimics the tumor microenvironment by including tumor, immune and stroll cells

DTCs Featured Resources

White Papers

DTCs Application: Checkpoint Inhibitor Therapy Utilization And PD1/PDL1 Expression Across A Global Clinical Network

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Protocols

View the standard process for thawing viable cell products from cryopreserved dissociated cell suspensions for optimal post-thaw yield.

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Presentations

Deep multi-omic phenotyping of biospecimens for immuno-oncology applications.

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DTCs Characterizations

Characterization Included on All DTCs:

  • Base Patient Data – Base patient data and redacted pathology report
  • Cell Populations – Cell population analysis by flow cytometry
  • HLA-A02 Status – HLA-A02 status by flow cytometry

Characterization Included on a subset of DTCs:

  • Targeted Genomic Analysis
    – A subset of our inventory is characterized by various genomic biomarkers (MSI, EGFR, KRAS, etc.) by PCR or NG
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Building a Targeted Cohort?

We can further characterize any DTCs samples via multi-omic biomarker services upon request to ensure your study cohorts are optimized for your biomarker targets.

DTCs Related Matched Sets

DTCs & Set Report

1000+

UNIQUE PATIENT
CASES AVAILABLE

  • This report includes cell population analysis and HLA-A02 typing by flow cytometry.
  • Mutation/biomarker tested patient samples are listed on a separate tab.

     

DTCs & FFPE

20+

UNIQUE PATIENT
CASES AVAILABLE

  • This report includes cell population analysis and HLA-A02 typing of the DTCs by flow cytometry.
  • Mutation/biomarker tested patient samples (PD-L1, MSI, MMR, BRAF, EGFR, KRAS, and NRAS) are listed on a separate tab within the report.

DTCs & PBMCs

200+

UNIQUE PATIENT
CASES AVAILABLE

  • This report includes cell population analysis and HLA-A02 typing of the DTCs by flow cytometry.
  • Mutation/biomarker tested patient samples (PD-L1, MSI, MMR, BRAF, EGFR, KRAS, and NRAS) are listed on a separate tab within the report.

DTCs & PBMCs + FFPE

200+

UNIQUE PATIENT
CASES AVAILABLE

  • This report includes cell population analysis and HLA-A02 typing of the DTCs by flow cytometry. 
  • Mutation/biomarker tested patient samples (PD-L1, MSI, MMR, BRAF, EGFR, KRAS, and NRAS) are listed on a separate tab within the report.
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Our Discovery Partners®  network allows for large-scale, prospective collections of high-quality matched sample sets of human biospecimens.

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Each dissociated tumor cell matched sample set is provided with the base clinical data from the patient case, along with cell population analysis and HLA-A02 typing by flow cytometry.

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Our scientific team has the knowledge and technical capacity to meet custom requirements and offer laboratory services for matched sample set projects.

Need biomarker characterization for your DTCs biospecimens?

While our biospecimens are highly characterized, we also provide streamlined multi-omic biomarker analyses to further characterize biospecimens from our inventory or from your studies via our global CLIA / CAP service laboratories.*

Sequencing

Genomic
Biomarkers

  • HudsonAlpha Discovery
  • Whole Genome Sequencing
  • Whole Exome Sequencing
  • Targeted Panels
  • Long-read Sequencing
  • RNASeq
  • Single Cell Sequencing
IHC

IHC
Services

  • Multiplexed & Singleplex IHC
  • In-Situ Hybridization
  • RNAScope
  • Digital Pathology & Image Analysis
  • PCR
  • Immunoflourescence
  • Colorimetric
Flow

Flow
Cytometry

  • Flow Cytometry
  • Dissociation & Isolation
  • Cell Culture & Expansion

*HudsonAlpha Discovery Sequencing & Bioinformatics laboratory is CLIA registered, pending CAP accreditation.

Learn More

Frequently Asked Questions

How are dissociated tumor cells (DTCs) generated? What protocol is used?
Following surgical resection, tumors are placed in a proprietary tissue storage solution and shipped in a NanoCool™ Cooling System to Discovery’s expert Custom Biospecimen Processing Laboratory. Upon receipt, tumors are minced into small pieces and undergo a proprietary mechanical and enzymatic digestion to the single cell level. This digestion takes approximately 1 hour. Following digestion, cells are washed and cryopreserved.
Are red blood cells lysed prior to cryopreservation?
Red blood cell lysis is not performed.
Are dead cells or debris removed prior to cryopreservation?
Dead cells or debris removal is not performed, as previous attempts at these protocols have only provided modest reductions in the number of dead cells or amount of debris while negatively impacting cellular yields and composition.
How are DTCs cryopreserved?
DTCs are cryopreserved in CryoStor© CS10.
What are the recommended storage conditions for the cryopreserved DTCs?
DTCs are shipped on dry ice. Upon receipt, they should be used immediately or placed in liquid nitrogen vapor phase for long term storage.
How are the cell counts and viabilities of DTCs determined?
Cell counts and viability are determined using a Nexcelom Cellometer® with acridine orange and propidium iodide to identify live and dead nucleated cells, respectively, per the recommendations provided by Nexcelom. Given the cellular debris that can be present, we do not recommend the use of trypan blue-based cell counting methods, as this will overestimate the number of dead cells.
What cell surface markers are cleaved during the dissociation process?
We have profiled the sensitivity of numerous cell surface markers to the enzymatic cocktail used to dissociate tumors. For inquiries on specific markers, please contact info@dls.com.
My cell surface marker of interest hasn’t been tested for sensitivity. Can you test it?
Yes, we are able to test receptor sensitivity using samples with known expression, such as PBMCs or cell lines. For more information, please contact info@dls.com.
My cell surface marker is cleaved during the dissociation process. Are modified protocols available?
Yes, modified dissociation protocols are available that may maintain the expression of sensitive cell surface markers. While expression is preserved, the overall viability of the sample may be negatively impacted. For more information, please contact info@dls.com.
Do you have any recommendations for culturing DTCs?

For short term cultures using DTCs, we recommend using ultra-low attachment plates, such as Corning™ Costar™ Ultra-Low Attachment Microplates. Conventional tissue culture-treated plates should be avoided. Serum-free media is also recommended, and we highly recommend using penicillin, streptomycin, gentamicin, primocin, and amphotericin B to limit bacterial and fungal growth, as sterility cannot be guaranteed. For more information, please contact info@dls.com.

Do you provide normal dissociated tissue cells or diseased dissociated tissue cells from indications other than cancer?

Yes, we have validated dissociations of certain normal tissue, including lung and colon. Autoimmune dissociated tissues are also available, including Crohn’s disease and ulcerative colitis. For more information, please tell us more about your project.

To propel your projects with The Power of Discovery.™

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