From the team who first validated cryopreserved tissue dissociations as a viable alternative to fresh cancer tissue samples for research applications.
Discovery’s expert cell services laboratory has evaluated DTCs for many applications through both internal R&D and client partnerships, cultivating unmatched expertise in applying cancer tissue dissociations to custom projects.
DTCs Product Details
- Guaranteed Cell Counts - Our Dissociated Tumor Cells (DTCs) and other tissue dissociations are delivered as cryopreserved vials of single cell suspensions with guaranteed cell counts following our Thawing Viable Cell Products protocol.
- Tumor, Immune & Stromal Cells – The final tissue dissociation vial will contain ALL of the various cell populations present in the tumor microenvironment.
- Proprietary Custom Protocols – With proprietary custom protocols, our scientists have consistently minimized the effects of cryopreservation and demonstrated high percentages of viable cells both pre-freeze (81.3%) and post-thaw (69.7%).
An ideal alternative to fresh tissues without the associated logistical challenges
High cell viability pre-freeze and post-thaw
Mimics the tumor microenvironment by including tumor, immune and stromal cells
DTCs Featured Resources
White Papers
DTCs Application: Checkpoint Inhibitor Therapy Utilization And PD1/PDL1 Expression Across A Global Clinical Network
Protocols
View the standard process for thawing viable cell products from cryopreserved dissociated cell suspensions for optimal post-thaw yield.
Presentations
Deep multi-omic phenotyping of biospecimens for immuno-oncology applications.
DTCs Characterizations
Characterization Included on All DTCs:
- Base Patient Data – Base patient data and redacted pathology report
- Cell Populations – Cell population analysis by flow cytometry
- HLA-A02 Status – HLA-A02 status by flow cytometry
Characterization Included on a subset of DTCs:
- Targeted Genomic Analysis
– A subset of our inventory is characterized by various genomic biomarkers (MSI, EGFR, KRAS, etc.) by PCR or NG
Building a Targeted Cohort?
We can further characterize any DTCs samples via multi-omic biomarker services upon request to ensure your study cohorts are optimized for your biomarker targets.
DTCs Related Matched Sets
DTCs & Set Report
1000+
UNIQUE PATIENT
CASES AVAILABLE
- This report includes cell population analysis and HLA-A02 typing by flow cytometry.
- Mutation/biomarker tested patient samples are listed on a separate tab.
DTCs & FFPE
20+
UNIQUE PATIENT
CASES AVAILABLE
- This report includes cell population analysis and HLA-A02 typing of the DTCs by flow cytometry.
- Mutation/biomarker tested patient samples (PD-L1, MSI, MMR, BRAF, EGFR, KRAS, and NRAS) are listed on a separate tab within the report.
DTCs & PBMCs
200+
UNIQUE PATIENT
CASES AVAILABLE
- This report includes cell population analysis and HLA-A02 typing of the DTCs by flow cytometry.
- Mutation/biomarker tested patient samples (PD-L1, MSI, MMR, BRAF, EGFR, KRAS, and NRAS) are listed on a separate tab within the report.
DTCs & PBMCs + FFPE
200+
UNIQUE PATIENT
CASES AVAILABLE
- This report includes cell population analysis and HLA-A02 typing of the DTCs by flow cytometry.
- Mutation/biomarker tested patient samples (PD-L1, MSI, MMR, BRAF, EGFR, KRAS, and NRAS) are listed on a separate tab within the report.
Our Discovery Partners® network allows for large-scale, prospective collections of high-quality matched sample sets of human biospecimens.
Each dissociated tumor cell matched sample set is provided with the base clinical data from the patient case, along with cell population analysis and HLA-A02 typing by flow cytometry.
Our scientific team has the knowledge and technical capacity to meet custom requirements and offer laboratory services for matched sample set projects.
Need Biomarker Characterization For Your DTCs biospecimens?
While our biospecimens are highly characterized, we also provide streamlined multi-omic biomarker analyses to further characterize biospecimens from our inventory or from your studies via our global CLIA / CAP service laboratories*.
Genomics
- HudsonAlpha Discovery
- Whole Genome Sequencing
- Whole Exome Sequencing
- Targeting Panels
- Hi-Fi Long Read Sequencing
- RNA Sequencing
- Single Cell Sequencing
Pathology
- IHC
- Multiplexed IF (mIF)
- RNA Scope
- Digital Pathology & Image Analysis
- PCR
- In-Situ Hybridization
- Colorimetric
Cell Biology
- Flow Cytometry
- Dissociation & Isolation
- Cell Culture & Expansion
Proteomics
- Proteogenomics
- Mass Spectrometry
- Multiplexed Immunoassays
*HudsonAlpha Discovery Sequencing and Bioinformatics laboratory is CLIA registered, pending CAP accreditation.
Learn More
Frequently Asked Questions
How are dissociated tumor cells (DTCs) generated? What protocol is used?
Are dead cells or debris removed prior to cryopreservation?
How are DTCs cryopreserved?
What are the recommended storage conditions for the cryopreserved DTCs?
How are the cell counts and viabilities of DTCs determined?
What cell surface markers are cleaved during the dissociation process?
My cell surface marker of interest hasn’t been tested for sensitivity. Can you test it?
My cell surface marker is cleaved during the dissociation process. Are modified protocols available?
Do you have any recommendations for culturing DTCs?
For short term cultures using DTCs, we recommend using ultra-low attachment plates, such as Corning™ Costar™ Ultra-Low Attachment Microplates. Conventional tissue culture-treated plates should be avoided. Serum-free media is also recommended, and we highly recommend using penicillin, streptomycin, gentamicin, primocin, and amphotericin B to limit bacterial and fungal growth, as sterility cannot be guaranteed. For more information, please contact info@dls.com.
Do you provide normal dissociated tissue cells or diseased dissociated tissue cells from indications other than cancer?
Yes, we have validated dissociations of certain normal tissue, including lung and colon. Autoimmune dissociated tissues are also available, including Crohn’s disease and ulcerative colitis. For more information, please tell us more about your project.
Are red blood cells lysed prior to cryopreservation?
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