Introduction:

The foundation of the future of biomedical research requires access to highly-annotated primary human biospecimens. The logistical barriers of acquiring fresh tissue remain an impediment to advances in medicine, requiring the coordination of not only the tissue collection but also the downstream applications in the laboratory. Dissociation and cryopreservation of solid tissue provide a solution to this problem. These single cell suspensions remain viable following cryopreservation and ease the demands on large-scale experimental assays. Furthermore, these cells provide the ability to screen new biomarkers and therapeutic targets as they are uncovered without the need to source new fresh tissue. Using this model, we have analyzed viably cryopreserved single cell suspensions generated from over … unique patients across .. oncology indications by flow cytometry. Flow cytometry allows for the identification of cell surface marker expression on the single cell level and provides indepth characterization of the cellular composition of the tumor microenvironment. This large-scale characterization revealed indication-specific trends to the tumor composition, which are vitally important considerations as the next-generation of therapeutic interventions are developed.

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