Get all the advantages of Discovery Fresh Leukopaks on your timeline

Prepared using optimized conditions and cryoprotectants, Discovery’s Cryo Leukopaks (RUO) provide all the same advantages as Discovery’s Fresh Leukopaks (RUO) — scale, consistency, deep-characterization, and speed—with the convenience of a frozen product. When thawed using our recommended protocol, you can achieve an industry-leading ~95% cell viability.

Key reasons why you should consider Discovery Cryo Leukopaks
  • Logistics & risk mitigation—Discovery Cryo Leukopaks allow your scientists to schedule their time, experiments, and downstream processes on their preferred schedule versus a fresh delivery schedule.
  • Deep characterization—Know the cell populations, HLA-Type, demographics, and real-time virus data when you select your Discovery Cryo Leukopak.
  • Same quality product—Discovery Cryo Leukopaks have the same cell population mix, viability, and cell counts as Discovery Fresh Leukopaks.
  • GMP-capable recallable donors—Collect a Discovery Cryo Leukopak, screen it, and have the same donor collected under GMP conditions.

Discovery’s Advantages


We have 2,800+ recallable donors that can support your research with consistent materials.


We can start collections within 2 – 14 days of receiving your purchase order, and provide same-day or next-day delivery, depending on your location.


We maintain a single chain of custody – from collection and leukapheresis to characterization and distribution of leukopaks to our clients. This affords consistent, rigorous quality control measures throughout and improves donor recallability via well-established donor relationships.


We are FDA Licensed for Blood Products, GMP/GLP compliant, CLIA Registered and CAP registered with accreditation pending

Advanced analysis and cell processing

The FDA licensed GMP Collection facility is housed in the same building as our Cell Processing, Flow Cytometry and our HudsonAlpha Discovery Genomics Center, which provides highly sophisticated analyses and high quality data meeting our customers’ turnaround time needs

Ready for GMP compliant leukopaks for clinical use?
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Highly characterized, recallable donors


mononuclear cells in every leukopak

<48 hours

from collection to delivery


viability post-thaw
On-Demand Video
Streamline Cell Therapy Development with High Quality Cellular Materials, In-House Apheresis Capabilities & Multi-Omics Analytical Services

Learn how to leverage our integrated platform to advance your cell therapies - watch this video of Dr. Scott Burger's presentation at the Allogeneic Cell Therapy Summit

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Detailed information

Discovery Cryo Leukopaks (RUO) are manufactured from the peripheral blood of normal human donors at our wholly owned and operated US Donor Center. They are frozen quickly after collection using a controlled rate method to maximize viability.

Each Discovery Cryo Leukopak (RUO) consists of a concentrated fraction of mononuclear cells including:
  • B cells
  • T cells
  • NK cells
  • Dendritic cells
  • Monocytes
  • CD34+

All cells are obtained using IRB approved consent forms and protocols. Each donor is tested for HIV, HBV, HCV, and CMV, and HLA typed via 6-digit NGS (download an example report).

Download an example report

Additional characterization can be performed— please inquire.

Immune Cell Populations in Discovery Leukopaks

Research Use Only. Not Intended for clinical use.

Discovery Cryo Leukopaks (RUO)Product No.Total Cell CountList Price*
Full Leukopak260240.019.5 Billion Minimum$6,864.00
Half Leukopak260260.015 Billion Minimum$4,680.00
Quarter Leukopak260280.012.5 Billion Minimum$2,912.00
Custom OrderCustomContact Us for Pricing

* Pricing may vary depending on your project specific details. Contact us for project specific pricing.

Donor information

All cells are obtained using IRB approved consent forms and protocols. We perform extensive characterization of all donors that includes:

Demographic information
  • Age
  • Height
  • Sex
  • Smoking status
  • Ethnicity
  • Blood type
  • Weight
HLA-typing to 6-digit resolution with NGS
Virus testing every 90 days
  • Hepatitis B Core Antibody (Anti-HBc EIA)
  • Hepatitis C Virus Antibody (Anti-HCV EIA)
  • Human Immunodeficiency Virus Antibody (HIV 1/2 plus O)
  • Additional tests can be performed— please inquire.

Frequently Asked Questions

How long until I receive my first shipment of Discovery Cryo Leukopaks?

Once we receive your purchase order, we can start collections within 2 – 14 days. Depending on your location, we provide same-day or next-day delivery.

Do you have a specific thawing protocol?

Watch a demonstration on our recommended method for handling and thawing.

What cryopreservation protocol do you follow?

We use a controlled rate method that quickly freezes leukopaks after collection to maximize viability, resulting in ~95% viability post-thawing.

Where are your sites?

Our Research Use Only (“RUO”) and our Good Manufacturing Processes (“GMP”) collections are both done in Huntsville, AL, in two separate suites in the same building. Our Cell Biology Lab is also co-located in the same facility, enabling real-time fresh processing or downstream manipulation as needed.

Your samples are all from healthy, normal donors—how do you define “normal"?

For a donor to qualify as normal, they cannot be currently diagnosed or have a history of cancer, autoimmune disease, or any major comorbidity (i.e. diabetes, high blood pressure, etc.). Contact us to learn more.

Can I follow the same donor from RUO to GMP?

Yes. Discovery Life Sciences owns and controls our entire supply chain from donor recruitment, donor screening, donor collection, and logistics to the end user. As a result, our operations are highly efficient and we can ensure a good relationship with our donors, with many returning again and again. Thus, you have the potential to use the same donor from RUO (Research Use Only) collections to GMP compliant collections for clinical use.

Has COVID-19 affected your collection, processing, and/or analytical facilities?

Our collection clinic, cell processing, NGS, and flow cytometry facilities have not been negatively impacted by COVID-19. We are located in Huntsville, Alabama, and have been considered an essential business by the state, enabling our facilities to remain open and fully operational. In addition, we have been able to continuously maintain our supply chain for lab consumables, keeping all projects running on schedule.

What apheresis equipment do you use?

We use Spectra Optia apheresis machines.

Which collection protocol/program and consumable sets are you using?

We use the CMNC (Continuous Mononuclear Cells) Protocol. This allows us to use only one separation process which is far more efficient than the MNC Protocol.

How often can you perform apheresis on a specific donor?

There must be a 61 day delay between one leukopheresis to the next donation. In addition, we require that our donors wait 21 days after a standard venipuncture (whole blood collection) before donating a leukopheresis product.

A Primer on Human PBMCs

A quick reference resource for scientists who need to isolate, manipulate or develop assays involving human mononuclear cells.

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Place your order or request project specific quote

Questions? Call our Customer Service Team at 1 (866) 838-2798.