SITC 2018 Poster
Exploring Dissociated Human Tissues as an Alternative to Fresh Tissue for Multiple Downstream Applications
by Shawn P. Fahl
November 2, 2018
The acquisition of fresh tissue is often an impediment to significant research advances. To provide an alternative to this issue, we have established cryopreserved dissociated human tissues as a viable specimen source with many applications. These single cell suspensions remain viable during the cryopreservation process and can be advantageous for multiple downstream applications, including immunophenotyping, cell culture, and sequencing, among others. Specifically, we have performed multiparametric flow cytometry on over 500 dissociated human tissues (tumor and normal) across 10 different oncology indications. As expected, we observed significant heterogeneity among the cellular composition for each individual tumor type. Across indications, however, trends were observed with regard to percentages of tumor and immune cell populations. Our data, case studies, and observed trends will be discussed.
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