Low-Input RNA Sequencing Pipeline Optimized to Minimize Sample Utilization

This technical note demonstrates the ability of Discovery Life Sciences to generate high-quality RNA sequencing data with high precision, accuracy, sensitivity, and specificity using minimal RNA input. Herein we evaluate multiple RNA input amounts, sourced from several different contrived reference and real-world clinical samples. We demonstrate that by utilizing our industry leading proprietary dual nucleic acid extraction method from FFPE samples coupled with our optimized low-input RNA-Seq library preparation pipeline yields high-quality RNA-Seq data with functional validity, thereby minimizing precious sample utilization and enabling more comprehensive, mullti-omic analyses.