Human Tissue Sample Blog

Changes in Peripheral Blood Over a Person's Lifetime

Posted by Luke Doiron on Aug 25, 2016 8:00:00 AM



Investigating the composition of peripheral blood of an individual can provide a window for both innate and outside experiences. While genetics will certainly influence composition, experiences like stress, disease, smoking and infection will also have an impact. Researchers studying cells isolated from peripheral blood donors need to be aware of the diverse influences that can affect their baseline. Some examples of these are outlined below.

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The Role of T Cells in Peripheral Blood Mononuclear Cells

Posted by Luke Doiron on Aug 18, 2016 8:00:00 AM


Peripheral blood mononuclear cells (PBMCs) are isolated from other cells types in human blood, most often by gradient centrifugation using Ficoll. Lymphocytes in the PBMC layer include T-cells, B-cells and NK-cells. Using certain immunological markers to identify the various populations of cells helps researchers determine the role of each cell type in the body’s disease defense system. T-cells are commonly separated into CD4+ (also know as helper T-cells) and CD8+ cells, which are cytotoxic. Further characterization of the roles of these important immune defense cells is currently a large area of investigation for researchers in a variety of different disease fields.

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Uses of Peripheral Blood Mononuclear Cells for Prostate Cancer Studies

Posted by Luke Doiron on Jun 9, 2016 8:00:00 AM


Prostate cancer is the second leading cause of cancer deaths in men globally, according to 2013 data. This fact spurs researchers to continue prostate cancer studies so they have a greater understanding of its carcinogenesis and progression, find reliable early diagnostic tools, and develop more effective and less toxic drug treatments.

Peripheral blood mononuclear cells (PBMCs) are isolated from whole blood and consist primarily of lymphocytes and monocytes, key to fighting disease. They have proven to be useful specimens for studying prostate cancer. PBMCs are typically provided to researchers as either fresh or cryopreserved specimens. Consider these applications of PBMCs for studying prostate cancer:

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Studying Mononuclear Cells for Cancer vs. Other Diseases

Posted by Luke Doiron on Jun 7, 2016 8:00:00 AM


Peripheral blood mononuclear cells (PBMCs) circulate in our blood and play an important role in immune system function, including recognition of foreign invaders such as cancer cells, viruses, or bacteria. PBMCs are blood cells with a round nucleus and are comprised of lymphocytes, including T and B cells, natural killer (NK) cells and monocytes, macrophages and dendritic cells. Researchers use PBMCs to study the mechanisms underlying immune disorders, cancer development, as well as identification of biomarkers in PBMCs that may point the way to better diagnostic warning tools for the early detection of cancer. Additionally, while mononuclear cells are most often studied for cancer and immune diseases, they also find application in other research areas.

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Considerations for Using Peripheral Blood for Breast Cancer Research

Posted by Luke Doiron on May 17, 2016 8:00:00 AM


With nearly all cancers, the earlier the diagnosis, the better the long-term prognosis. For breast cancer, in particular, the good news is that survival rates are steadily improving, thanks to increased awareness and the widespread use of mammogram screening. Yet, the number of deaths estimated for 2016 is expected to exceed 40,000, a number that is still unacceptably high. One key reason is time of diagnosis and cancer stage at diagnosis. Statistics from the American Cancer Society find that the five-year survival for stage I breast cancer (2012) was 100%, but only 22% for stage IV metastatic breast cancer. These numbers continue to add urgency to the search for better diagnostic, prognostic and treatment tools.

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5 Types of Mononuclear Cells and How They Differ

Posted by Luke Doiron on May 10, 2016 8:00:00 AM



Every day, we encounter billions of  unseen enemies - bacteria, viruses, and fungi - ready to attack and make us ill. Fortunately, our immune system has evolved into a lean, mean fighting machine, and when in proper working order, it routinely sends out a variety of warriors to fight off and defeat bacterial and viral threats such as a nasty cold or flu. These immune system components are part of our peripheral blood mononuclear cells (PBMC), which are basically blood cells with round nuclei.

Though there are two main types of mononuclear cells (lymphocytes and monocytes), there are 5 key subtypes within the two main categories. Each one has a specific and important role to play in fighting infection, cancerous cells, and other foreign intruders.

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Tips for Maximizing Peripheral Blood Mononuclear Cell Viability

Posted by Rachel Lane on Apr 26, 2016 8:00:00 AM


Peripheral blood mononuclear cells (PBMCs) have a variety of useful research applications, and researchers can isolate fresh PBMCs from whole blood samples or thaw cryopreserved isolated PBMCs for their studies. Fresh and cryopreserved biospecimens must be handled properly to maintain cell viability and ensure reliable results. Below are tips for processing whole blood, isolating PBMCs, and thawing cryopreserved PBMC samples to achieve optimal cell integrity for subsequent experiments. 

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How to Avoid Peripheral Blood Mononuclear Cell Aggregation

Posted by Rachel Lane on Apr 7, 2016 7:00:00 AM



Cryopreserved peripheral blood mononuclear cells (PBMCs) offer a variety of research applications and have been used in many studies, including cancer and autoimmune disease research. Proper thawing procedures must be followed to ensure the maximum recovery, viability and functionality of cryopreserved PBMCs. Improper sample thawing induces cell lysis, which releases DNA and causes viable cells to aggregate.1 Commercial products have been developed to increase post-thaw PBMC viability, and combining these products with optimized thawing techniques improves the integrity of PBMC samples. 

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