Human Tissue Sample Blog

The 4 Best Cell Isolation Kits to Perform Tissue Dissociations

Posted by Luke Doiron on Aug 13, 2015 1:15:00 PM

    

Separation of viable, high-quality cells from tumor tissue or other types of biospecimens is a critical step in biomedical research and development. The results of your research are impacted in part by the quality of the starting biospecimen as well as the methodology used to dissociate the tissues and isolate cells found in that sample. Properly prepared cell mixtures that are high in viability, purity and yield give you a positive jump-start for quality yields of RNA, proteins and other cell structures; these in turn are invaluable for downstream uses such as isolating and identifying cancer biomarkers or gene expression analysis.

Read More

What Parameters Impact the Outcome of Viable Dissociated Cells

Posted by Luke Doiron on Jul 29, 2015 3:11:00 PM

    

When cell isolation is the goal, selecting the right protocol can greatly influence cell viability and yield, and therefore impact your research data. This is why it's important to understand what parameters effect the outcome of cell dissociation processes, so you select the correct materials and methods for your desired endpoint and downstream application.

Read More

7 Steps for Successful Basic Primary Cell Isolation

Posted by Luke Doiron on Jul 22, 2015 2:07:00 PM

    

 

Primary cell isolation is required for subsequent generation of primary cell cultures, which are useful in-vitro tools for pre-clinical disease research. Common applications of primary cell isolates include studies of cellular communication and illumination of disease mechanisms, including cancer, autoimmune diseases, and diabetes.

Primary cells from a biospecimen are typically heterogeneous, so basic cell isolation is used to dissociate and separate the various cell types from tissue specimens. Many researchers believe that primary cells best represent the tumor biology and heterogeneity encountered in the actual clinical environment, and so are worthwhile and important in-vitro models.

When it comes to basic primary cell isolation, it's key to understand that there are a wide variety of parameters that can impact the outcome of any isolation procedure. These include the type of tissue, species origin, specimen age, genetic modification (i.e. knockouts), enzyme type and quality, and incubation time and temperature.

Read More

8 Types of Enzymes for Tumor Cell Dissociation

Posted by Luke Doiron on Jul 7, 2015 12:00:00 PM

    

Cancer researchers understand that there is quite a bit of heterogeneity among the cells found in human tumor biospecimens. Such variations can result in cells that differ in growth properties, drug toxicity, immunological reactivity, and proliferative potential. For this reason, when tumor specimens are used for in-vitro studies, it's very important to properly isolate and dissociate all the cells from one another, so that researchers gain better understanding of tumor progression or regression properties, as well as predicting therapeutic responses to novel treatment modalities, including potential new drug regimens.

Read More

5 Best Practices for Working with Enzymes for Tissue Dissociation

Posted by Luke Doiron on Jun 3, 2015 10:03:39 AM

    

Biomedical research frequently relies on high-quality tissue specimens that are then enzymatically dissociated into single cell preparations. These cells are useful for a wide range of studies, such as gene expression profiles or analysis of cell surface markers, as well as for growing cell cultures using an isolated primary cell. Successful tissue dissociation with enzymes is affected by a variety of factors which can impact cell viability, yield, and ultimately, clinical usefulness.

Read More

4 Techniques for Dissociation of Human Cancer Cells

Posted by Luke Doiron on Jun 2, 2015 3:48:40 PM

    


Over the years, a variety of protocols and methods have been developed for dissociation of different types of human cancer cells. One reason for the rise of different protocols was a 1989breast cancer study that found a link between the types of cancer cellular subpopulations found in dissociated tumor samples and the method of dissociation used.

Read More

Subscribe to the Conversant Bio blog

New Call-to-action