Cryopreservation is widely used for stabilizing and storing biological materials such as tissue specimens at very low (cryogenic) temperatures. Advancements in understanding the best methods for cryopreservation, thawing and culturing of biological specimens have enabled such samples to be stored for many years and still be viable specimens for studying a variety of cellular processes.
Determining the best method for culture of thawed cryopreserved samples is an important step in many research protocols. Depending on the research goal and tissue specimen, culture methods will vary to some degree. Peripheral blood mononuclear cells (PBMCs), whole tumor tissue, and bone marrow are just a few examples of the cryopreserved samples that scientists use for culturing.
One important point repeatedly mentioned in the literature is that cryopreservation must be done correctly. Tissue samples should be frozen in 10% DMSO, never snap frozen, without temperature fluctuation during storage time. In particular, specimens should remain below the glass transition point to maximize long-term utility.
The following culture protocols have been used for cryopreserved samples.Read More