Human Tissue Sample Blog

Rachel Lane

Recent Posts

Tips for Maximizing Peripheral Blood Mononuclear Cell Viability

Posted by Rachel Lane on Apr 26, 2016 8:00:00 AM

    

Peripheral blood mononuclear cells (PBMCs) have a variety of useful research applications, and researchers can isolate fresh PBMCs from whole blood samples or thaw cryopreserved isolated PBMCs for their studies. Fresh and cryopreserved biospecimens must be handled properly to maintain cell viability and ensure reliable results. Below are tips for processing whole blood, isolating PBMCs, and thawing cryopreserved PBMC samples to achieve optimal cell integrity for subsequent experiments. 

Read More

How to Avoid Peripheral Blood Mononuclear Cell Aggregation

Posted by Rachel Lane on Apr 7, 2016 7:00:00 AM

    

 

Cryopreserved peripheral blood mononuclear cells (PBMCs) offer a variety of research applications and have been used in many studies, including cancer and autoimmune disease research. Proper thawing procedures must be followed to ensure the maximum recovery, viability and functionality of cryopreserved PBMCs. Improper sample thawing induces cell lysis, which releases DNA and causes viable cells to aggregate.1 Commercial products have been developed to increase post-thaw PBMC viability, and combining these products with optimized thawing techniques improves the integrity of PBMC samples. 

Read More

Methods for Coculturing Cancer Cells with Immune Cells

Posted by Rachel Lane on Aug 27, 2015 10:46:00 AM

    

Communication among cells in the tumor microenvironment can promote or inhibit cancer growth. To determine how proximal cell populations affect tumor progression, cells of interest are often cultured together in a shared space in vitro. This "coculturing" method simulates the tumor microenvironment and can be used to reveal how tumor cells influence noncancerous cell populations and vice versa. In addition, thoughtfully designed coculturing experiments can reveal if the observed effects are mediated either directly, through physical cell-cell interactions, or indirectly, through soluble proteins. 

Read More

Considerations for Optimizing Peripheral Blood Mononuclear Cell Cultures

Posted by Rachel Lane on Apr 14, 2015 9:31:00 AM

    

Peripheral blood mononuclear cells (PBMCs), which mainly include lymphocytes and monocytes, can be isolated from whole blood for use in a variety of in vitro applications, such as cell culture. Isolated PBMCs are used in cell culture experiments to investigate the molecular mechanisms underlying diseases, such as cancer, and to screen and develop potential therapeutics. Optimized cell culture conditions improve result reproducibility, providing valuable insight into disease pathology, prevention, and treatment. Key considerations for optimizing cell culture experiments with isolated PBMCs are summarized below.

Read More

Four Downstream Applications for Isolated Peripheral Blood Mononuclear Cells

Posted by Rachel Lane on Nov 25, 2014 6:00:00 AM

    

 

Peripheral blood mononuclear cells (PBMCs), which mainly include lymphocytes (i.e., T cells, B cells, and natural killer [NK] cells) and monocytes, are a valuable research tool. This cell population is usually isolated from whole blood via simple density gradient centrifugation, and fresh or cryopreserved PBMCs can be used for studies. PBMCs are a versatile cell population with applications in multiple research fields, including those listed below. 

Read More

Rheumatoid Arthritis Risk Factors and Stages

Posted by Rachel Lane on Sep 9, 2014 6:00:00 AM

    

Rheumatoid arthritis (RA) is a chronic immune-mediated inflammatory disease that most commonly occurs in the proximal interphalangeal and metacarpophalangeal joints of the hand, in the wrists, and in small foot joints but can also affect shoulders, knees, elbows, and ankles. Three times more women than men develop RA, and 40- to 60-year-old individuals are most commonly diagnosed with this diease. The underlying cause of RA is not well understood, but most researchers believe that a combination of genetic and nongenetic factors are involved. Several of these risk factors and the general disease pathology are discussed below.

Read More

Subscribe to the Conversant Bio blog

New Call-to-action