Science

Discovery scientists were the first to validate cryopreserved tissue dissociations as a viable alternative to sourcing fresh tissue for many research applications. With proprietary custom protocols, our scientists have consistently demonstrated high percentages of viable cells both pre-freeze (81.3%) and post-thaw (69.7%). We also have found that most cell types are largely unaffected by cryopreservation. Click to access the white paper confirming cell viability in Dissociate Tumor Cells (DTCs) via flow cytometry.

Our team conducted a flow cytometry analysis of over 400 tumor dissociations across 11 oncology indications to observe indication-specific trends in tumor compositions. The data from this large-scale flow cytometry analysis of tumor dissociations has been presented at scientific meetings. 

We continually innovate the utility of tissue dissociations in contemporary research and downstream applications (Box 1). Leveraging sophisticated technologies and techniques, including flow cytometry and genomic applications, we are expanding our sample characterizations and laboratory services. Exploring these applications in R&D and through client partnerships, our team has cultivated a deep expertise in applying tissue dissociations to custom projects. We are available for consultations to optimize the results of studies involving our tissue dissociations.

Box 1. Research Applications

  • Flow Cytometry
  • Sequencing
  • Cell Isolation Studies
  • Cell Culturing
  • 3D Organoids
  • Patient-Derived Xenografts (PDX)
  • Identifying Novel Biomarkers
  • Translational Development
  • Monitoring Immune Activation
  • Drug Response and Target Discovery

Service

Our tissue dissociations are delivered as cryopreserved vials of single cell suspensions with guaranteed cell counts following our Thawing Viable Cell Products protocol. Specimens are processed using validated procedures that have been optimized per disease indication. Each specimen is provided with the base clinical data from the patient case, along with a redacted pathology report.

Characterizations:

Our scientific team continually develops deeper characterization levels for our dissociated tissue inventories. Existing inventories are not retroactively characterized, so our inventory is segmented by level of characterization. Click the links below to access inventory sheets for tissue dissociations by characterization level:

  • Base Patient Data - includes only base patient data and redacted pathology report
  • Cell Populations - includes cell population analysis by flow cytometry + base patient data
  • HLA-A02 StatusHLA-A02 status by flow cytometry + cell populations + base patient data
  • Targeted Genomic Analysis - various biomarker and mutational statuses (MSI, EGFR, KRAS, etc.) by PCR or sequencing, without regard for characterization level
 
 
 
 
 
 
 
 
 
Related Services:

Sophisticated technologies and resourceful scientists are available to alleviate pressure on internal resources and accelerate projects timelines. Services related to dissociated tissue samples include:


Connect With Us:

Submit this form to start a conversation with Discovery to:

  • Begin a dissociated tissues purchase
  • Discuss a custom project
  • Ask questions about our biospecimens