Target Expression
Unique integrated biospecimen and expert biomarker services enable a thorough understanding of target expression.
Study Design & Cohort Creation
- Define specific tissue characteristics desired
- Develop a strategy for a cohort of low prevalence biomarkers
- Select FFPE specimens matching study criteria from our bioStore or initiate a prospective collection using our global clinical site network of Discovery Partners
- Identify proper controls
- Create custom FFPE positive and negative controls verified by flow cytometry
- Optimized dual DNA/RNA extraction services
- Orthogonal testing via RNAScope, PCR, ELISA, Nanostring, etc.
- Genomic biomarker analyses: RNA-Seq, whole exome sequencing and targeted sequencing, whole genome sequencing, long read sequencing, single cell and more
- Immunophentyping and functional analyses via flow cytometry and cell-based services
Understand Target Expression
- Conduct large scale sensitivity screening of cancer patient populations to understand expression patterns within and among tumor indications
- Demonstrate precision and reproducibility using serial sections from samples with various levels of known target expression
- Analyze specificity in cell lines, xenografts, and normal human tissues
- Optimized dual DNA/RNA extraction services
- Develop a robust scoring scheme to properly and consistently capture level of target expression
- Analyze expression rate for cut-off determination in various disease indications
- Orthogonal testing via RNAScope, PCR, ELISA, Nanostring, etc.
- Genomic biomarker analyses: RNA-Seq, whole exome sequencing and targeted sequencing, whole genome sequencing, long read sequencing, single cell and more
- Immunophentyping and functional analyses via flow cytometry and cell-based services
Case Study: Pembrolizumab Protoype Assay
See Our Industry-Leading Assay Validation Approach
Precision & Reproducibility: See An Example
See Our Industry-Leading Assay Validation Approach
To propel your projects with The Power of Discovery.™
Contact Us Today
Let us know about your project!
