TruSight Oncology 500 (TSO500) ctDNA NGS Services

Reduce time to develop and validate your oncology liquid biopsy research and diagnostic assay development by utilizing our TSO500 ctDNA application to detect ctDNA genomic variants and biomarker signatures

Learn MoreContact Us
HudsonAlpha Discovery’s TSO500 ctDNA NGS service provides high sensitivity detection of genomic variants across 523 DNA genes in various cancer indications from liquid biopsy plasma samples, including microsatellite instability (MSI) and tumor mutational burden (TMB) immuno-oncology biomarkers.
For Research Use Only.
An On Demand Webinar

High Sensitivity TSO500 Detection of Somatic Variants in Matched FFPE and Plasma: Implications for Liquid Biopsy Biomarker Evaluations

Presented by Shawn Levy, PhD (Discovery Life Sciences)

Watch this webinar to learn how Discovery leverages TSO500 sequencing capabilities to conduct a comparative analysis of paired plasma and FFPE samples, and more.

Watch Webinar


Our TSO500 NGS services provide pan-cancer genomic biomarker analysis to enable:
  • Discovery and verification of the presence of genomic biomarker targets and signatures in liquid biopsy ctDNA
  • Retrospective identification of potential oncology clinical trial patient responders versus non-responders based on genomic profiles
  • RUO development and orthogonal validation of novel liquid biopsy diagnostic solutions
  • Combine the matching TSO500 ctDNA and TSO500 Solid Tumor applications to develop and validate non-invasive liquid biopsy assays from matched-patient Plasma and FFPE samples

Service Performance Data

Using commercially available reference standards in our optimized and automated nucleic acid extraction, library construction, and sequencing workflows, we demonstrate the accuracy, sensitivity, reproducibility, and concordance of our matched liquid biopsy ctDNA – solid tumor TSO500 NGS assay service.



Limit of Detection:

>1% Allele Fractions



ctDNA / Solid Tumor Concordance:


Technical Data Sheet

Download our Technical Data sheet to learn more about service performance data.

TSO500 Technical Data Sheet Download Now


HudsonAlpha Discovery’s TSO500 ctDNA NGS services provide results via a convenient and comprehensive PierianDx report for genomic variant, TMB, and MSI scores.

Horizon OncoSpan cfDNA Reference Standard Sample Report View Sample Report
Horizon OncoSpan Solid Tumor gDNA Reference Standard Sample View Sample Report
The following output files are also available in addition to FASTQ and BAM files.

TS500 ctDNA v1.0.1 Output files

DNA Results
  • Small variants VCF
  • Annotated merged variants JSON
  • TMB trace TSV
QC and metrics files
  • Metrics report TSV

Sample Input Requirements


Blood collection tubes should be EDTA or Streck tubes, no Heparin tubes allowed

Plasma samples should be collected and separated within 2 hours of collection if possible as longer times impact degradation and downstream sequencing success
Double spun plasma is also acceptable

1 - 2 mL plasma frozen at -80°C with no signs of hemolysis

ctDNA extractions from Plasma will be done using the Beckman Coulter Apostle MiniMax ctDNA Extraction Kit with automated extractions performed on the Beckman i7 robot

ctDNA QC will be performed on the Fragment Analyzer

ctDNA Input: >30 ng in 50 ul volume

NGS sequencing runs using paired end 2 x 150 base pair reads

Start your project today

or learn more about how we can advance your liquid biopsy biomarker discovery and development programs

Select your matched FFPE and plasma cohorts

Utilize our optimized NGS Services

Understand results without bioinformatics services