HudsonAlpha Discovery’s TSO500 ctDNA NGS service provides high sensitivity detection of genomic variants across 523 DNA genes in various cancer indications from liquid biopsy plasma samples, including microsatellite instability (MSI) and tumor mutational burden (TMB) immuno-oncology biomarkers.
— For Research Use Only —
AN ON DEMAND WEBINAR
High Sensitivity TSO500 Detection of Somatic Variants in Matched FFPE and Plasma: Implications for Liquid Biopsy Biomarker Evaluations
APPLICATIONS
Our TSO500 NGS services provide pan-cancer genomic biomarker analysis to enable:
- Discovery and verification of the presence of genomic biomarker targets and signatures in liquid biopsy ctDNA
- Retrospective identification of potential oncology clinical trial patient responders versus non-responders based on genomic profiles
- RUO development and orthogonal validation of novel liquid biopsy diagnostic solutions
- Combine the matching TSO500 Solid Tumorand TSO500 ctDNA applications to develop and validate non-invasive liquid biopsy assays from matched-patient Plasma and FFPE samples
Service Performance Data
Using commercially available reference standards in our optimized and automated nucleic acid extraction, library construction, and sequencing workflows, we demonstrate the accuracy, sensitivity, and reproducibility of our TSO500 Solid Tumor NGS RUO service.
Accuracy:
> 99%
Limit of Detection:
> 1% Allele Fractions
Reproducibility:
> 99%
ctDNA / Solid Tumor Concordance:
> 99%
Technical Data Sheet
Download our Technical Data sheet to learn more about service performance data.
TSO500 Technical Data Sheet
Deliverables
HudsonAlpha Discovery’s TSO500 ctDNA NGS services provide results via a convenient and comprehensive PierianDx report for genomic variant, TMB, and MSI scores.
Horizon OncoSpan cfDNA Reference Standard Sample Report
Horizon OncoSpan Solid Tumor gDNA Reference Standard Sample
THE FOLLOWING OUTPUT FILES ARE ALSO AVAILABLE IN ADDITION TO FASTQ AND BAM FILES.
TS500 ctDNA v1.0.1 Output files
DNA Results
- Small variants VCF
- Annotated merged variants JSON
- CNV VCF
- TMB trace TSV
QC and metrics files
- Metrics report TSV
Sample Input Requirements
- Blood collection tubes should be EDTA or Streck tubes, no Heparin tubes allowed
- Plasma samples should be collected and separated within 2 hours of collection if possible as longer times impact degradation and downstream sequencing success. Double spun plasma is also acceptable.
- 1 - 2 mL plasma frozen at -80°C with no signs of hemolysis
- ctDNA extractions from Plasma will be done using the Beckman Coulter Apostle MiniMax ctDNA Extraction Kit with automated extractions performed on the Beckman i7 robot
- ctDNA QC will be performed on the Fragment Analyzer
- ctDNA Input: >30 ng in 50 ul volume
- NGS sequencing runs using paired end 2 x 150 base pair reads
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